MicroRNA-218 inhibits gastrointestinal stromal tumor cell and invasion by targeting KIT

摘要: The objectives of this study were to detect the expressions microRNA-218 (miR-218) in human gastrointestinal stromal tumor (GIST) tissues and cells explore its effects on biological features GIST-T1 expression target gene KIT, so as provide new insights for GIST treatment. Using quantitative real-time polymerase chain reaction (qRT-PCR), we detected miR-218 adjacent cell lines including GIST882, GIST430, GIST48, GIST-T1. Forty-eight hours after mimic was transfected into cells, by qRT-PCR. effect viability using MTT. proliferation apoptosis analyzed flow cytometry. Transwell invasion chamber applied cells. KIT identified be a luciferase reporter enzyme system, protein determined Western blotting. As shown qRT-PCR, compared with that tissue, tissue significantly decreased (P < 0.0001). Compared control group, increased 48 h (P < 0.01). MTT showed overexpression Flow cytometry index declined (P < 0.01); meanwhile, also Detection number passing through dropped enhanced Luciferase assay that, relative activity transfection group In conclusion, decreases lines. can negatively regulate inhibit Treatment based may promising strategy GIST.