Establishment of Patient‐Derived Xenograft (PDX) Models of Human Breast Cancer

摘要: Patient-derived xenograft (PDX) models of human breast cancer are proving useful for preclinical evaluation experimental therapeutics. However, until recently, generation PDX reflecting the full spectrum cancers has been an elusive goal. We recently developed a method establishing serially transplantable, phenotypically stable, in immunocompromised mice with comparatively high efficiency (overall ∼25%). These xenografts represent major clinically defined subtypes [e.g. estrogen receptor positive (ER+), HER2 (HER2+), and "triple negative" (TN) cancers]. This method, methods being other laboratories, may soon allow conducting "animal clinical trials" once sufficient numbers relevant generated. Curr. Protoc. Mouse Biol. 3:21-29 © 2013 by John Wiley & Sons, Inc.