Naturally derived micelles for rapid in vitro screening of potential cholesterol-lowering bioactives.

摘要: A high plasma cholesterol level, especially low-density lipoprotein cholesterol, indicates increased risk of cardiovascular diseases. Plasma levels are influenced by diet and biosynthesis, uptake, secretion. Cholesterol uptake involves solubilization into complex phospholipid spherical bodies termed micelles that facilitate the transport lipids through gut brush border membrane enterocytes. In vitro assays reported to date determine potential cholesterol-lowering effects various compounds require artificial micelle preparations elaborate time-consuming prepare. The aims this study were compare efficacy artificially prepared with naturally derived from pig's bile test their ability assess inhibitors uptake. suitability bile-derived was tested both at level cellular using cultured Caco-2 cells. Known (green tea catechins) cell (beta-lactoglobulin-derived peptide, IIAEK) used as reference inhibitory compounds. It concluded a rapid, reproducible, convenient, cost-effective source for solubility assay systems is suitable screening purposes focused on identifying agents.