Tissue tropisms of porcine parvovirus in swine

摘要: Late-term gestation swine fetuses, similar to adult animals, are able effectively mount immune response and survive porcine parvovirus (PPV) infection. An exception this is the Kresse strain of PPV, which causes fetal death in late-term fetuses. In an effort understand basis for profound difference pathogenicity between prototype NADL-8, studies were designed examine potential sites replication quantity virus produced NADL-8 strains. order define viral or sequestration situ hybridization, using digoxigenin-labeled strand-specific oligonucleotide probes, was applied detect presence either double stranded DNA (RF) single tissues infected The state confirmed by Southern blot hybridization. Relative amounts RF-DNA synthesis each tissue compared two Virus appeared be comparable levels livers both Differences these strains observed brain spleen; detected spleens fetuses but not ones. These findings indicate that differences PPV selective sites, as well produced, may explain distinction pathogenesis viruses.