Co-Expression of ORFCma with PHB Depolymerase (PhaZCma ) in Escherichia coli Induces Efficient Whole-Cell Biodegradation of Polyesters.
作者： Ming-Chieh Lee , En-Jung Liu , Cheng-Han Yang , Li-Jung Hsiao , Tzong-Ming Wu
关键词:
摘要: Whole-cell degradation of polyesters not only avoids the tedious process enzyme separation, but also allows degraded product to be reused as a carbon source. In this study, Escherichia coli BL21(DE3) harboring phaZCma , gene encoding poly(3-hydroxybutyrate) (PHB) depolymerase from Caldimonas manganoxidans, is constructed. The extra-cellular fraction E. coli/pPHAZ exhibits fast PHB rate where it took 35 h completely degrade films, while C. manganoxidans takes 81 h do same. co-expression ORFCma (a putative periplasmic substrate binding protein that within same operon ) further improves degradation. While 28 h needed for cause an 80% weight loss in coli/pORFPHAZ needs 21 h. Furthermore, able at-least four different polyesters, PHB, poly(lactic acid) (PLA), polycaprolactone (PCL), and poly(butylene succinate-co-adipate) (PBSA). Testing time course 3-hydroxybutyrate concentration turbidity solutions over shows PhaZCma has both exo- endo-enzymatic activity. whole-cell can used recycling various potentially universal element enhancing secretion recombinant protein.
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