Role of Mechanical Stress-induced Glutamate Signaling-associated Molecules in Cytodifferentiation of Periodontal Ligament Cells
作者： Chiharu Fujihara , Satoru Yamada , Nobuhiro Ozaki , Nobuo Takeshita , Harumi Kawaki
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摘要: In this study, we analyzed the effects of tensile mechanical stress on gene expression profile in vitro-maintained human periodontal ligament (PDL) cells. A DNA chip analysis identified 17 up-regulated genes PDL cells under stress, including HOMER1 (homer homolog 1) and GRIN3A (glutamate receptor ionotropic N-methyl-d-aspartate 3A), which are related to glutamate signaling. RT-PCR real-time PCR analyses revealed that constitutively expressed signaling-associated increased these mRNAs, leading release from intracellular signal transduction. Interestingly, exogenous mRNAs cytodifferentiation mineralization-related as well ALP (alkaline phosphatase) activities during On other hand, signaling inhibitors riluzole (+)-MK801 maleate suppressed alkaline phosphatase mineralized nodule formation mineralization. Riluzole inhibited stress-induced expressions Moreover, situ hybridization showed up-regulation at tension sites orthodontic tooth movement a mouse model. The present data demonstrate induced by positively regulates mineralization
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