Hypoxia regulates the proliferation and osteogenic differentiation of human periodontal ligament cells under cyclic tensile stress via mitogen-activated protein kinase pathways.
作者： Lu Li , Min-xuan Han , Sheng Li , Yan Xu , Lin Wang
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摘要: Background: Previous studies have shown that periodontal ligament exists in a hypoxic microenvironment, especially under the condition of periodontitis or physical stress. The present study is designed to investigate effects and mechanisms hypoxia on regulating proliferation osteogenic differentiation human cells (hPDLCs) cyclic tensile stress (CTS).Methods: hPDLCs were cultured 2% O2 (hypoxia) 20% (normoxia) then subjected in-plane deformation 10% at 0.5 Hz. following parameters measured: 1) cell by flow cytometry; 2) ultrastructure transmission electron microscopy; 3) expression hypoxia-inducible factor-1α (HIF-1α) relative factors (i.e., secreted phosphoprotein 1 [SPP1; also known as bone sialoprotein I/osteopontin], runt-related transcription factor 2 [RUNX2], Sp7 [SP7]) real-time polymerase chain reaction Western blot; 4) involvement ...
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