Identification and integrated analysis of differentially expressed lncRNAs and circRNAs reveal the potential ceRNA networks during PDLSC osteogenic differentiation

摘要: Researchers have been exploring the molecular mechanisms underlying control of periodontal ligament stem cell (PDLSC) osteogenic differentiation. Recently, long noncoding RNAs (lncRNAs) and circular (circRNAs) were shown to function as competitive endogenous (ceRNAs) regulate effect microRNAs (miRNAs) on their target genes during However, comprehensive identification integrated analysis lncRNAs circRNAs acting ceRNAs PDLSC differentiation not performed. PDLSCs derived from healthy human cultured separately with induction normal media for 7 days. Cultured positive STRO-1 CD146 negative CD31 CD45. Osteo-induced showed increased ALP (alkaline phosphatase) activity up-regulated expression levels osteogenesis-related markers ALP, Runt-related transcription factor 2 osteocalcin. Then, a total 960 1456 found be differentially expressed by RNA sequencing. The profiles eight measured quantitative real-time polymerase chain reaction agree RNA-seq results. Furthermore, potential functions predicted based miRanda investigated using Gene Ontology Kyoto Encyclopedia Genes Genomes analysis. In total, 147 1382 combine 148 common miRNAs compete miRNA binding sites 744 messenger RNAs. These mRNAs significantly participate in osteoblast differentiation, MAPK pathway, Wnt pathway signaling pathways regulating pluripotency cells. Among them, coded TCONS_00212979 TCONS_00212984, well circRNA BANP ITCH, might interact miRNA34a miRNA146a via pathway. This study comprehensively identified lncRNAs/circRNAs first ceRNA findings suggest that specific promote regeneration.