Heme Binding Constricts the Conformational Dynamics of the Cytochrome b(559)' Heme Binding Cavity
作者： Yasar Akdogan , Veerappan Anbazhagan , Dariush Hinderberger , Dirk Schneider , None
DOI: 10.1021/BI300489S
关键词:
摘要: Cytochrome b(559)' is a transmembrane protein formed by homodimerization of the 44-residue PsbF polypeptide and noncovalent binding heme cofactor. The can dimerize in absence presence heme. To monitor structural alterations associated with to apo-cytochrome, we analyzed apo- holo-cytochrome structure electron paramagnetic resonance spectroscopy. Spin labeling amino acids located close domain cytochrome revealed that unconstrained Heme restricts conformational dynamics domain, resulting structurally more constricted structure.
mpg.de
core.ac.uk
acs.org
sci-hub.se 参考文章(47)
Wayne L. Hubbell, David S. Cafiso, Christian Altenbach, Identifying conformational changes with site-directed spin labeling. Nature Structural & Molecular Biology. ,vol. 7, pp. 735- 739 ,(2000) , 10.1038/78956
Thomas Volkmer, Christian Becker, Alexander Prodöhl, Carmen Finger, Dirk Schneider, Assembly of a transmembrane b-Type cytochrome is mainly driven by transmembrane helix interactions Biochimica et Biophysica Acta. ,vol. 1758, pp. 1815- 1822 ,(2006) , 10.1016/J.BBAMEM.2006.05.009
Alexander Prodöhl, Thomas Volkmer, Carmen Finger, Dirk Schneider, Defining the structural basis for assembly of a transmembrane cytochrome. Journal of Molecular Biology. ,vol. 350, pp. 744- 756 ,(2005) , 10.1016/J.JMB.2005.05.016
S.C.T. Nicklisch, D. Wunnicke, I.V. Borovykh, S. Morbach, J.P. Klare, H.-J. Steinhoff, R. Krämer, Conformational changes of the betaine transporter BetP from Corynebacterium glutamicum studied by pulse EPR spectroscopy. Biochimica et Biophysica Acta. ,vol. 1818, pp. 359- 366 ,(2012) , 10.1016/J.BBAMEM.2011.10.021
Ruth Horn, Harald Paulsen, Early steps in the assembly of light-harvesting chlorophyll a/b complex: time-resolved fluorescence measurements. Journal of Biological Chemistry. ,vol. 279, pp. 44400- 44406 ,(2004) , 10.1074/JBC.M407188200
Sandip Shinde, Jeanine M. Cordova, Brian W. Woodrum, Giovanna Ghirlanda, Modulation of function in a minimalist heme-binding membrane protein Journal of Biological Inorganic Chemistry. ,vol. 17, pp. 557- 564 ,(2012) , 10.1007/S00775-012-0876-1
Jeanine M. Cordova, Pamela L. Noack, Simon A. Hilcove, James D. Lear, Giovanna Ghirlanda, Design of a functional membrane protein by engineering a heme-binding site in glycophorin A. Journal of the American Chemical Society. ,vol. 129, pp. 512- 518 ,(2007) , 10.1021/JA057495I
Alexander Prodöhl, Mathias Weber, Carolin Dreher, Dirk Schneider, A mutational study of transmembrane helix–helix interactions Biochimie. ,vol. 89, pp. 1433- 1437 ,(2007) , 10.1016/J.BIOCHI.2007.06.006
Hans-Georg Koch, Dirk Schneider, Folding, Assembly, and Stability of Transmembrane Cytochromes Current Chemical Biology. ,vol. 1, pp. 59- 74 ,(2007) , 10.2174/2212796810701010059
Yiqing Feng, Stephen G. Sligar, Effect of heme binding on the structure and stability of Escherichia coli apocytochrome b562. Biochemistry. ,vol. 30, pp. 10150- 10155 ,(1991) , 10.1021/BI00106A011