The phylogenetic utility of chloroplast and nuclear DNA markers and the phylogeny of the Rubiaceae tribe Spermacoceae

摘要: Abstract The phylogenetic utility of chloroplast (atpB-rbcL, petD, rps16, trnL-F) and nuclear (ETS, ITS) DNA regions was investigated for the tribe Spermacoceae coffee family (Rubiaceae). ITS was, despite often raised cautions its at higher taxonomic levels, shown to provide highest number parsimony informative characters, in partitioned Bayesian analyses it yielded fewest trees 95% credible set, resolved proportion well clades, most accurate region as measured by partition metric correctly clades (well supported retrieved from a combined analysis regarded “true”). For Hedyotis, 5S-NTS be potentially useful ITS, shorter sequence length. being phylogenetically petD group II intron. We also present phylogeny based on four regions, ETS combined. are monophyletic. Clades high posterior probabilities discussed, especially respect current generic classification. Notably, Oldenlandia is polyphyletic, two subgenera Kohautia not sister taxa, Hedyotis should treated narrow sense include only Asian species.