Tissue Engineering of Functional Salivary Gland Tissue

摘要: Objectives: Treatment of conditions that lead to salivary hypofunction, such as radiation induced xerostomia, is currently limited the administration saliva substitutes and sialogogues. The transient effect these medications necessitates frequent administration, systemic side effects may be intolerable. creation implantable, functional gland tissue from autologous glandular cells would provide a physiologic solution this problem. We investigated feasibility engineering in vivo. Methods: Primary human were grown, expanded, seeded on biodegradable polymer scaffolds. A total 48 scaffolds with 16 control polymers without implanted subcutaneously athymic mice. implants retrieved 2, 4, 8 weeks after implantation for phenotypic analyses. Results: Human epithelial retained their characteristics at all culture stages. Histologically, formation acinar gland-like structures was observed within engineered by 4 implantation. Immunocytochemical Western blot analyses tissues demonstrated expression a-amylase, cytokeratins AE1/ AE3, aquaporin-5 using cell-specific antibodies. Reverse-transcription polymerase chain reaction confirmed type α-amylase (σ-amylase) mRNA. production over time biochemical amylase detection system.