Characterisation of PigC and HapC, the prodigiosin synthetases from Serratia sp. and Hahella chejuensis with potential for biocatalytic production of anticancer agents

摘要: PigC and HapC catalyse the condensation of 4-methoxy-2,2′-bipyrrole-5-carbaldehyde (MBC) with 2-methyl-3-amylpyrrole (MAP) to give bright red tripyrrolic prodigiosin, which has potent anticancer activity. We have cloned over-expressed both enzymes characterised their enzymic activity in vitro using natural substrates, MBC MAP, analogues these substrates. Thus a range prodigiosin been produced. Both are membrane-associated attempts fully solubilise them detergents led inactivation. The ATP-dependent but, unlike they show greatest similarity, by-product is ADP not AMP. Two different slowly interconverting rotamers exist spectral changes time consistent isomerisation E,Z (or α) rotamer Z,Z β) rotameric form.