Chemical hybridizing agent SQ-1-induced male sterility in triticum aestivum L.: a comparative analysis of the anther proteome.

摘要: Heterosis is widely used to increase the yield of many crops. However, as wheat a self-pollinating crop, hybrid breeding not so successful in this organism. Even though male sterility induced by chemical hybridizing agents an important aspect crossbreeding, mechanisms which these induce well understood. We performed proteomic analyses using Triticum aestivum L.to identify those proteins involved physiological (PHYMS) agent CHA SQ-1. A total 103 differentially expressed were found 2D–PAGE and subsequently identified MALDI-TOF/TOF MS/MS. In general, had obvious functional tendencies implicated carbohydrate metabolism, oxidative stress resistance, protein photosynthesis, cytoskeleton cell structure. combination with phenotypic, tissue section, bioinformatics analyses, revealed complex network behind regulation PHYMS pollen development. Accordingly, we constructed wheat, drawing relationships between their annotated biological pathways. To further validate our proposed network, determined relevant values real-time PCR assays. Our proteomics based approach has enabled us certain anthers. Anomalies metabolism stress, together premature tapetum degradation, may be cause starvation SQ-1 treated plants. Here, provide insight into underlying SQ-1-induced sterility. findings have practical implications for application wheat.