Irradiation inhibits the maturation and mineralization of osteoblasts via the activation of Nrf2/HO-1 pathway
作者： Sung-Ho Kook , Kyoung-A Kim , Hyeok Ji , Daewoo Lee , Jeong-Chae Lee
DOI: 10.1007/S11010-015-2559-Z
关键词:
摘要: Nuclear factor (erythroid-derived 2)-like 2 (Nrf2) regulates the induction of antioxidant gene expression and protects cells against oxidative injury. However, there are controversial findings regarding roles Nrf2 on bone metabolism under stress. The role differentiation radiation-exposed osteoblasts is also unclear. We investigated whether negatively or positively affects osteoblast in response to irradiation. Irradiation inhibited MC3T3-E1 a dose-dependent manner. This inhibition was evidenced by irradiation-mediated decreases bone-like nodule formation, alkaline phosphatase (ALP) activity, calcium accumulation, markers, such as ALP, osteocalcin, osteopontin, sialoprotein, osterix, Runx2. These reductions were accompanied increased heme oxygenase-1 (HO-1), accumulation cellular oxidants, depletion defense enzymes. siRNA-mediated silencing markedly reversed negative effect irradiation cells, leading decrease HO-1 an increase Runx2 levels. Irradiation-mediated levels osteocalcin mRNA, but not protein, significantly inhibitor, zinc protoporphyrin IX. Furthermore, N-acetyl cysteine restored all changes induced near-normal cells. results demonstrate that inhibits mineralization through stress-mediated activation Nrf2/HO-1 pathway.
springer.com
sci-hub.se 参考文章(42)
Nariman Panahian, Mahin D. Maines, The heme oxygenase system and cellular defense mechanisms Advances in Experimental Medicine and Biology. ,vol. 502, pp. 249- 272 ,(2001) , 10.1007/978-1-4757-3401-0_17
A. Dare, R. Hachisu, A. Yamaguchi, S. Yokose, S. Yoshiki, T. Okano, Effects of Ionizing Radiation on Proliferation and Differentiation of Osteoblast-like Cells Journal of Dental Research. ,vol. 76, pp. 658- 664 ,(1997) , 10.1177/00220345970760020601
Salih Ozgocmen, Huseyin Kaya, Ersin Fadillioglu, Rabia Aydogan, Zumrut Yilmaz, Role of antioxidant systems, lipid peroxidation, and nitric oxide in postmenopausal osteoporosis Molecular and Cellular Biochemistry. ,vol. 295, pp. 45- 52 ,(2007) , 10.1007/S11010-006-9270-Z
Young-Ok Son, Sung-Ho Kook, Ki-Choon Choi, Yong-Suk Jang, Young-Mi Jeon, Jong-Ghee Kim, Kyung-Yeol Lee, Ju Kim, Mi-Sun Chung, Gook-Hyun Chung, Jeong-Chae Lee, Quercetin, a bioflavonoid, accelerates TNF-α-induced growth inhibition and apoptosis in MC3T3-E1 osteoblastic cells European Journal of Pharmacology. ,vol. 529, pp. 24- 32 ,(2006) , 10.1016/J.EJPHAR.2005.10.041
Soon-Sun Park, Kyoung-A Kim, Seung-Youp Lee, Shin-Saeng Lim, Young-Mi Jeon, Jeong-Chae Lee, X-ray radiation at low doses stimulates differentiation and mineralization of mouse calvarial osteoblasts Journal of Biochemistry and Molecular Biology. ,vol. 45, pp. 571- 576 ,(2012) , 10.5483/BMBREP.2012.45.10.101
Chris Wambi, Jenine Sanzari, X. Steven Wan, Manunya Nuth, James Davis, Ying-Hui Ko, Carly M. Sayers, Matthew Baran, Jeffrey H. Ware, Ann R. Kennedy, Dietary Antioxidants Protect Hematopoietic Cells and Improve Animal Survival after Total-Body Irradiation Radiation Research. ,vol. 169, pp. 384- 396 ,(2008) , 10.1667/RR1204.1
Shizu Hirata, Chiaki Kitamura, Hidefumi Fukushima, Ikuo Nakamichi, Yoshimitsu Abiko, Masamichi Terashita, Eijiro Jimi, Low-level laser irradiation enhances BMP-induced osteoblast differentiation by stimulating the BMP/Smad signaling pathway Journal of Cellular Biochemistry. ,vol. 111, pp. 1445- 1452 ,(2010) , 10.1002/JCB.22872
Ann D. King, James F. Griffith, Jill M. Abrigo, Sing-fai Leung, Fung-kwai Yau, Gary M.K. Tse, Anil T. Ahuja, Osteoradionecrosis of the upper cervical spine: MR imaging following radiotherapy for nasopharyngeal carcinoma European Journal of Radiology. ,vol. 73, pp. 629- 635 ,(2010) , 10.1016/J.EJRAD.2008.12.016
Peng Jia, You Jia Xu, Zeng Li Zhang, Kai Li, Bingyan Li, Wen Zhang, Huilin Yang, Ferric ion could facilitate osteoclast differentiation and bone resorption through the production of reactive oxygen species. Journal of Orthopaedic Research. ,vol. 30, pp. 1843- 1852 ,(2012) , 10.1002/JOR.22133
Jung Sun Heo, Seung-Youp Lee, Jeong-Chae Lee, Wnt/β-catenin signaling enhances osteoblastogenic differentiation from human periodontal ligament fibroblasts. Molecules and Cells. ,vol. 30, pp. 449- 454 ,(2010) , 10.1007/S10059-010-0139-3