Lidocaine potentiates the chondrotoxicity of methylprednisolone.

摘要: Purpose This study examined the viability of bovine articular chondrocytes after exposure to methylprednisolone, methylprednisolone with lidocaine, and in a simulated inflammatory environment. Methods Bovine were suspended alginate beads cultured Dulbecco's modified Eagle's medium/F-12 for 1 week before experimentation. Suspended exposed 0.9% saline solution (negative control), (4, 8, 16 mg/mL), (8 mg/mL) 1% or environment (interleukin [IL] 1β exposure, 10 ng/mL) 15, 30, 60 minutes. Flow cytometry was performed day, 4 days, 7 days by use annexin V propidium iodide assess chondrocyte viability. Results Chondrocyte decreased from 84% 62%, 38%, 2.4% day minutes 4, mg/mL respectively (n = 7, P Conclusions These results show dose- time-dependent decrease clinically relevant doses methylprednisolone. The combination lidocaine toxic, virtually no cells surviving treatment. In addition, did not mitigate effects IL-1β; rather, it further potentiated chondrotoxicity. Clinical Relevance Intra-articular injections corticosteroids local anesthetics are widely used clinical practice. vitro provides information on potential these drugs cartilage.