Determination of D- and L-alanine concentrations using a pyruvic acid sensor.

摘要: The concentrations of D- and L-alanine in bivalves are useful as indicators environmental pollution. Amino acid oxidase with a low substrate specificity catalyzes the oxidation various amino acids. Among acids, pyruvic can be generated from alanine only by catalytic oxidative reaction this oxidase. Therefore, study, were determined concentration acid, which was consumption oxygen based on pyruvate From point view, there is very strong possibility that biosensors utilizing enzymes developed. results obtained follows. (1) optimum conditions for use sensor follows: temperature 25 degrees C, pH 6.8, flow rate 0.1 ml/min, thiamin diphosphate 1.5 mM, injection volume 50 microl. (2) D-Alanine optimally reacted L-amino at 30 8.2, min 37 7.8, 90 min, respectively. (3) linear relationships between output 3.56-106.8 microg D-alanine 5.34-71.3 L-alanine. (4) Meretrix iusoria, Patinopecten yessonsi, Corbicula leana proposed assay good agreement those conventional method.