A Small Molecule Inhibitor of Tgf-β Signaling Replaces Sox2 in Reprogramming by Inducing Nanog

摘要: The combined activity of three transcription factors can reprogram adult cells into induced pluripotent stem (iPSCs). However, the transgenic methods used for delivering reprogramming have raised concerns regarding future utility resulting cells. These uncertainties could be overcome if each factor were replaced with a small molecule that either directly activated its expression from somatic genome or in some way compensated activity. To this end, we high-content chemical screening to identify molecules replace Sox2 reprogramming. We show one these functions by inhibiting Tgf-β signaling stable and trapped intermediate cell type forms during process. find inhibition promotes completion through induction Nanog.