A simple LC-MS method for determination of cyasterone in rat plasma: application to a pilot pharmacokinetic study.
作者： Fuqiang Li , Guangyu Li , Jinsong Zhao , Jun Xiao , Zaoxia Liu
DOI: 10.1002/BMC.3621
关键词:
摘要: A simple, specific, and sensitive liquid chromatography-mass spectrometry (LC-MS) method for determination of cyasterone in rat plasma was developed our laboratory. Cucurbitacin B used as an internal standard (IS). After protein precipitation with twofold volume acetonitrile, the analyte IS were separated on a Luna C18 column (100 × 4.6 mm, i.d., 3.0 µm; Phenomenex) by isocratic elution acetonitrile-water (80:20, v/v) mobile phase at flow rate 0.4 mL/min. An electrospray ionization source applied operated positive ion mode; selected monitoring scan mode quantification, target ions m/z 543.3 581.3 chosen. Good linearity observed concentration range 0.40-400 ng/mL plasma. Intra-day inter-day precision both <7.4%. This proved to be suitable pharmacokinetic studies after oral (5.0 mg/kg) or intravenous (0.5 administration rats. Copyright © 2015 John Wiley & Sons, Ltd.
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