Effect of leukocyte antibodies and HLA matching on the intravascular recovery, survival, and tissue localization of 111-indium granulocytes

摘要: The effect of leukocyte antibodies detected under different conditions on the fate in vivo granulocytes was studied using 111-indium-labeled granulocytes. Sera from patients were tested by granulocyte agglutination (GA), granulocytotoxicity (GC), immunofluorescence (GIF), lymphocytotoxicity (LC), and antibody-dependent lymphocyte-mediated granulocytotoxicity. Granulocytes donors to be labeled with 111-indium injected. Then intravascular recovery survival or tissue localization determined 93 studies. Antibodies associated a significant reduction (6.7% v 30.8% controls; P less than .001) t1/2 (0.3 hours 5.6 = .002). When all possible combinations serum reactivity considered, GA plus GIF assays had best correlation decreased (R2 .49; .73; .001). relationship between strength antibody analyzed, combination LC .62; Because general availability HLA (LC) testing, role investigated other ways. There strong sera highly reactive those GIF. These also reduced t1/2. influence specific antigen mismatches studied. donor recipient mismatched for HLA-A2, B8, BW44 antigens, there either recovery, t1/2, both. Tissue body scans without known sites inflammation. caused abnormal pulmonary sequestration (three cases) failure localize at inflammation cases). did not alter despite presence corresponding antigens It appears that GA, GIF, these tests is most effective predictor altered However, probably define group immunized whom are reduced. Mismatching certain survival. At present, assay, activity.(ABSTRACT TRUNCATED AT 400 WORDS)