Xanthohumol Inhibits Notch Signaling and Induces Apoptosis in Hepatocellular Carcinoma

摘要: Despite improvement in therapeutic strategies, median survival advanced hepatocellular carcinoma (HCC) remains less than one year. Therefore, molecularly targeted compounds with toxic profiles are needed. Xanthohumol (XN), a prenylated chalcone has been shown to have anti-proliferative effects various cancers types vitro. XN treatment healthy mice and humans yielded favorable pharmacokinetics bioavailability. we determined study the of understand mechanism its action HCC. The on panel HCC cell lines were assessed for viability, colony forming ability, cellular proliferation. Cell lysates analyzed pro-apoptotic (c-PARP cleaved caspase-3) anti-apoptotic markers (survivin, cyclin D1, Mcl-1). concentrations 5μM above significantly reduced ability also confluency all four studied. Furthermore, growth suppression due apoptosis was evidenced by increased expression proteins. Importantly, inhibited Notch signaling pathway as decrease Notch1 HES-1 Ectopic cells reverses effect compared control. Taken together these results suggested that mediated is appeared be inhibition pathway. our findings warrants further studies potential agent