Differentially expressed genes induced by cold and UV-B in Deschampsia antarctica Desv.

作者: Jorge Dinamarca , Alejandra Sandoval-Alvarez , Manuel Gidekel , Ana Gutiérrez-Moraga

DOI: 10.1007/S00300-012-1271-7

关键词:

摘要: Antarctica is one of the most extreme environments on Earth. Deschampsia antarctica Desv. only monocot vascular plant that colonizes Antarctic Peninsula. The survival this species in harsh environment suggests possesses genes associated with cold and UV tolerance. Using suppression subtractive hybridization, we identified a total 112 differentially expressed under irradiance conditions. Northern blot analysis real-time RT-PCR confirmed expression differences among several genes. similarity search analysis, number have not been previously reported. results showed radiation mainly induce related to transcription, energy defense response. Interestingly, part isolated corresponds unknown or hypothetical proteins. This set tolerance-related could be relevant uncover mechanisms by which extremophile survives its contribute development biotechnology species.

参考文章(56)
J.G. Scandalios, Oxidative stress: molecular perception and transduction of signals triggering antioxidant gene defenses Brazilian Journal of Medical and Biological Research. ,vol. 38, pp. 995- 1014 ,(2005) , 10.1590/S0100-879X2005000700003
C. L. Guy, K. J. Niemi, R. Brambl, Altered gene expression during cold acclimation of spinach. Proceedings of the National Academy of Sciences of the United States of America. ,vol. 82, pp. 3673- 3677 ,(1985) , 10.1073/PNAS.82.11.3673
Irena Gielwanowska, Ewa Szczuka, New ultrastructural features of organelles in leaf cells of Deschampsia antarctica Desv Polar Biology. ,vol. 28, pp. 951- 955 ,(2005) , 10.1007/S00300-005-0024-2
S Altschula, Warren Gisha, Webb Millerb, E Meyersc, D Lipmana, None, Basic Local Alignment Search Tool Journal of Molecular Biology. ,vol. 215, pp. 403- 410 ,(1990) , 10.1016/S0022-2836(05)80360-2
F. S. Xiong, Christopher T. Ruhland, Thomas A. Day, Photosynthetic temperature response of the Antarctic vascular plants Colobanthus quitensis and Deschampsia antarctica Physiologia Plantarum. ,vol. 106, pp. 276- 286 ,(1999) , 10.1034/J.1399-3054.1999.106304.X
Makoto Hashimoto, Setsuko Komatsu, Proteomic analysis of rice seedlings during cold stress Proteomics. ,vol. 7, pp. 1293- 1302 ,(2007) , 10.1002/PMIC.200600921
Manuel Gidekel, Luis Destefano-Beltr�n, Patricia Garc�a, Lorena Mujica, Pamela Leal, Marely Cuba, Lida Fuentes, Le�n A. Bravo, Luis J. Corcuera, Miren Alberdi, Ilona Concha, Ana Guti�rrez, Identification and characterization of three novel cold acclimation-responsive genes from the extremophile hair grass Deschampsia antarctica Desv. Extremophiles. ,vol. 7, pp. 459- 469 ,(2003) , 10.1007/S00792-003-0345-4
Liang Chen, Hui Zhong, Feng Ren, Qian-Qian Guo, Xu-Peng Hu, Xue-Bao Li, A novel cold-regulated gene, COR25, of Brassica napus is involved in plant response and tolerance to cold stress. Plant Cell Reports. ,vol. 30, pp. 463- 471 ,(2011) , 10.1007/S00299-010-0952-3
Xiao-yan Xu, Rui Fan, Rui Zheng, Chun-mei Li, De-yue Yu, Proteomic analysis of seed germination under salt stress in soybeans Journal of Zhejiang University SCIENCE B. ,vol. 12, pp. 507- 517 ,(2011) , 10.1631/JZUS.B1100061
Takashi Hirayama, Kazuo Shinozaki, Research on plant abiotic stress responses in the post‐genome era: past, present and future Plant Journal. ,vol. 61, pp. 1041- 1052 ,(2010) , 10.1111/J.1365-313X.2010.04124.X