A lineage-specific centromeric satellite sequence in the genus Trifolium.

作者: Helal A. Ansari , Nick W. Ellison , Andrew G. Griffiths , Warren M. Williams

DOI: 10.1023/B:CHRO.0000034099.19570.B7

关键词:

摘要: We report the molecular structure, genomic organization, chromosomal distribution and evolutionary dynamics of TrR350, a satellite DNA isolated from forage legume white clover (Trifolium repens L.; 2n = 4x= 32). The basic repeating unit is an A+T rich 350 bp HindIII fragment with complex dimeric structure consisting internal direct repeat 156 packed between unrelated flanking sequences. Each has conserved 24 motif at two places. Most short units enclose pentanucleotide CAAAA motif, presumed to be involved in breakage-reunion mechanism tandemly arrays. dimers share high sequence homology among themselves while monomers within show significant divergence. Genomic Southern hybridization and/or fluorescence situ (FISH) on 17 Trifolium species/subspecies revealed that it lineage-specific confined several species section Lotoidea originating Mediterranean region. uniform length centromeric localization most harbouring reflects its extensive conservation lineage. However, restriction profile seven also indicated independent evolution this repeat.

参考文章(35)
Robert J. Shmookler Reis, Arun Srivastava, David T. Beranek, Samuel Goldstein, Human alphoid family of tandemly repeated DNA. Sequence of cloned tetrameric fragments and analysis of familial divergence. Journal of Molecular Biology. ,vol. 186, pp. 31- 41 ,(1985) , 10.1016/0022-2836(85)90254-2
M. Morgante, I. Jurman, L. Shi, T. Zhu, P. Keim, J. A. Rafalski, The STR120 satellite DNA of soybean: organization, evolution and chromosomal specificity. Chromosome Research. ,vol. 5, pp. 363- 373 ,(1997) , 10.1023/A:1018492208247
M. J. Baker, W. M. Williams, White clover taxonomy and biosystematics. CAB International. pp. 323- 342 ,(1987)
J. S. Heslop-Harrison, Andrea Brandes, Trude Schwarzacher, Tandemly repeated DNA sequences and centromeric chromosomal regions of Arabidopsis species. Chromosome Research. ,vol. 11, pp. 241- 253 ,(2003) , 10.1023/A:1022998709969
Joan Pons, Carlos Juan, Eduard Petitpierre, None, Higher-order organization and compartmentalization of satellite DNA PIM357 in species of the coleopteran genus Pimelia Chromosome Research. ,vol. 10, pp. 597- 606 ,(2002) , 10.1023/A:1020918803675
S. W. Hussain, W. M. Williams, Development of a fertile genetic bridge between Trifolium ambiguum M. Bieb. and T. repens L. Theoretical and Applied Genetics. ,vol. 95, pp. 678- 690 ,(1997) , 10.1007/S001220050612
W Williams, Evidence of Three Subspecies in Trifolium nigrescens Viv. Annals of Botany. ,vol. 87, pp. 683- 691 ,(2001) , 10.1006/ANBO.2001.1399
Helal A Ansari, NIick W Ellison, Steve M Reader, Ekaterina D Badaeva, Bernd Friebe, Terry E Miller, Warren M Williams, Molecular Cytogenetic Organization of 5S and 18S-26S rDNA Loci in White Clover (Trifolium repens L.) and Related Species Annals of Botany. ,vol. 83, pp. 199- 206 ,(1999) , 10.1006/ANBO.1998.0806
JM Zhu, NW Ellison, RW Rowland, Chromosomal localization of a tandemly repeated DNA sequence in Trifolium repens L. Cell Research. ,vol. 6, pp. 39- 46 ,(1996) , 10.1038/CR.1996.5