Effects of amino acids, epinephrine and heparin upon the radioimmunoassay of insulin in plasma.

摘要: Recent reports have indicated that substances such as arginine, leucine, epinephrine and heparin artifactually alter the radioimmunoassay measurement of insulin. To further evaluate this problem fifty-eight plasma samples were enriched with leucine 141 epinephrine, all assayed for insulin by a double antibody technic. Addition these resulted in no alteration measurements. Arginine, did not standard solutions human Also, 114 pairs heparinplasma serum insulin, but significant differences noted. Heparin also had effect on It is concluded capable specifically measuring levels presence or epinephrine. appears either are equally suitable determinations. Comparison our data published from other laboratories indicate there specificity methods used various laboratories, each laboratory must validate its own procedure.