Herpes simplex viruses and herpes B virus.

摘要: This chapter focuses on the herpes simplex viruses (HSV) and B virus, describing their transmission, clinical significance, detection mechanisms. Herpes virus types 1 2 (HSV-1 HSV-2), formally designated human herpesvirus 2, respectively, are members of family Herpesviridae. Primary infection with HSV-1 or HSV-2 is followed by establishment latency in dorsal root ganglia, typically trigeminal ganglia for orolabial disease lumbosacral genital disease. Immunostaining methods to detect antigen require less expertise than cytopathic effect (CPE)-based culture usually expensive culture. Polymerase chain reaction (PCR) provides best sensitivity direct approaches. Tests based crude mixtures still marketed, but they have unacceptably low specificity, especially detecting new infections those prior infection. The HSV Western blot assay used University Washington laboratory uses nitrocellulose blots prepared diploid fibroblast-infected cell proteins. blotting detects antibodies multiple viral proteins, including type-specific glycoproteins gG-1 gG-2. Simple gG-based lateral-flow assays available that designed point-of-care testing. Type-specific serology critical identifying pregnant women infections. Serodiagnosis has been complicated extensive cross-reactivity HSV-2. Serologic testing can be useful evaluation potentially infected animals involved exposures screening research animals.