Replication of polyoma virus DNA: I. A resting cell system for biochemical studies on polyoma virus
作者： Mike Fried , John D. Pitts
DOI: 10.1016/0042-6822(68)90097-4
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摘要: Abstract A technique is described for adapting mouse embryo cells to the resting state by using medium supplemented with only 0.5% serum. In maintained in low serum 4 days rates of DNA and RNA syntheses, activities polymerase DNase, are very low. large proportion (90%) such can be infected polyoma virus at an input multiplicity 20 PFU/cell. After infection there increases syntheses activity, but DNase activity remains
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