Production and Evaluation of an Avian IgY Immunotoxin against CD133+ for Treatment of Carcinogenic Stem Cells in Malignant Glioma: IgY Immunotoxin for the Treatment of Glioblastoma.

摘要: Background. Glioblastoma is the most common malignant tumor of Central Nervous System. Despite research in therapeutics, prognosis dismal. Malignant glioma stem cells (MGSCs) are a major cause treatment failure and increasing recurrence. In general, cancer (CSCs) express prominin-1 (CD133), considered as potential therapeutic target. this study, we produced an avian immunotoxin directed against subpopulation CD133+ CSCs within glioma. We used IgY because it has various advantages increased affinity to mammal antigens inexpensive obtention large amounts specific antibodies (approximately 1 mg/per egg). The design, production, purification use anti CD133 constitute original goal research. Methods. immunodominant peptide was designed immunize hens; also, extracellular domain cloned probe antibodies. parallel, recombinant abrin A chain E. coli order join Fc anti-CD133 conform immunotoxin. This anti-tumor tested vitro vivo. Results. cytotoxicity showed that IgY-abrin reduced 55% cell viability. After subcutaneous MGSCs implantation, animals treated intraperitoneally or intratumorally with more than 50% decrease volume. Conclusion. Results had cytotoxic activity provides novel approach for immunotherapy glioblastoma.