Lymphocytes induce monocyte chemoattractant protein-1 production by renal cells after Fcγ receptor cross-linking: role of IL-1β

摘要: Leukocyte recruitment to the kidney in immune complex disease like systemic lupus erythematosus (SLE) is mediated part by local expression of chemokines such as monocyte chemoattractant protein-1 (MCP-1). Recent studies from this laboratory demonstrated that cross-linking Fc gammaR on lymphocytes causes release a soluble factor induces chemokine production. To explain induction renal disease, we postulated lymphocyte stimulates parenchymal cell MCP-1 expression. test hypothesis, human peripheral blood were incubated immobilized IgG, model for cross-linking. Supernatants these cultures significantly increased production mesangial, glomerular capillary endothelial, and proximal tubular epithelial cells. Mesangial cells IgG or with soluble, preformed complexes did not secrete above control levels. Lymphocyte supernatant-induced appeared be dependent presence interleukin (IL)-1beta supernatant. Removing IL-1beta supernatants, antagonizing its activity, preventing conversion mature abrogated supernatants. These data demonstrate response gammaR, induce secreting IL-1beta. Renal may thus triggered traffic through encounter deposited complexes.