miR-330-5p suppresses glioblastoma cell proliferation and invasiveness through targeting ITGA5
作者： Linsen Feng , Jianhua Ma , Haiming Ji , Yichun Liu , Weixing Hu
DOI: 10.1042/BSR20170019
关键词:
摘要: The present study intended to investigate the biological effects of miR-330-5p on glioblastoma (GBM) cell proliferation and invasiveness by targeting integrin α5 (ITGA5). expressions ITGA5 mRNA in GBM lines (U87, U251, U373) normal brain glial line (HEB) were detected using RT-qPCR. Protein expression was examined Western blot. used MTT assay, colony formation Transwell wound healing flow cytometry analysis order determine functions cells (including proliferation, invasion, migration, apoptosis, cycle). applied dual-luciferase reporter gene assay identify target relationship between ITGA5. low-expressed while high-expressed compared with HEB. could directly as well suppress its cells. Up-regulation down-regulation both have an inhibitory effect migration. Meanwhile, they also promote apoptosis. invasion through
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David P Bartel, MicroRNAs: Genomics, Biogenesis, Mechanism, and Function Cell. ,vol. 116, pp. 281- 297 ,(2004) , 10.1016/S0092-8674(04)00045-5
Solange Tréhoux, Fatima Lahdaoui, Yannick Delpu, Florence Renaud, Emmanuelle Leteurtre, Jérôme Torrisani, Nicolas Jonckheere, Isabelle Van Seuningen, Micro-RNAs miR-29a and miR-330-5p function as tumor suppressors by targeting the MUC1 mucin in pancreatic cancer cells. Biochimica et Biophysica Acta. ,vol. 1853, pp. 2392- 2403 ,(2015) , 10.1016/J.BBAMCR.2015.05.033
Michel J. Weber, New Human and Mouse microRNA Genes Found by Homology Search FEBS Journal. ,vol. 272, pp. 59- 73 ,(2004) , 10.1111/J.1432-1033.2004.04389.X
Xiang Zhang, Shu-Li Cheng, Ka Bian, Lei Wang, Xiao Zhang, Bo Yan, Lin-Tao Jia, Jing Zhao, Noor Gammoh, An-Gang Yang, Rui Zhang, MicroRNA-26a promotes anoikis in human hepatocellular carcinoma cells by targeting alpha5 integrin. Oncotarget. ,vol. 6, pp. 2277- 2289 ,(2015) , 10.18632/ONCOTARGET.2956
Jennifer Lake, Karine Zaniolo, Marie-Ève Gingras, Camille Couture, Christian Salesse, Sylvain L. Guérin, Functional Impact of Collagens on the Activity Directed by the Promoter of the α5 Integrin Subunit Gene in Corneal Epithelial Cells. Investigative Ophthalmology & Visual Science. ,vol. 56, pp. 6217- 6232 ,(2015) , 10.1167/IOVS.15-16587
Jian-Jun Xie, Jin-Cheng Guo, Zhi-Yong Wu, Xiu-E Xu, Jian-Yi Wu, Bo Chen, Li-Qiang Ran, Lian-Di Liao, En-Min Li, Li-Yan Xu, Integrin α5 promotes tumor progression and is an independent unfavorable prognostic factor in esophageal squamous cell carcinoma Human Pathology. ,vol. 48, pp. 69- 75 ,(2016) , 10.1016/J.HUMPATH.2015.09.029
Joachim Silber, C. David James, J. Graeme Hodgson, microRNAs in Gliomas: Small Regulators of a Big Problem NeuroMolecular Medicine. ,vol. 11, pp. 208- 222 ,(2009) , 10.1007/S12017-009-8087-9
Yuefeng Li, Xiaolan Zhu, Wenlin Xu, Dongqing Wang, Jinchuan Yan, miR-330 regulates the proliferation of colorectal cancer cells by targeting Cdc42. Biochemical and Biophysical Research Communications. ,vol. 431, pp. 560- 565 ,(2013) , 10.1016/J.BBRC.2013.01.016
R Schickel, B Boyerinas, S-M Park, M E Peter, MicroRNAs: key players in the immune system, differentiation, tumorigenesis and cell death Oncogene. ,vol. 27, pp. 5959- 5974 ,(2008) , 10.1038/ONC.2008.274
Y Murakami, T Yasuda, K Saigo, T Urashima, H Toyoda, T Okanoue, K Shimotohno, Comprehensive analysis of microRNA expression patterns in hepatocellular carcinoma and non-tumorous tissues Oncogene. ,vol. 25, pp. 2537- 2545 ,(2006) , 10.1038/SJ.ONC.1209283