In vitro growth and differentiation of osteoblast- like human bone marrow cells on glass reinforced hydroxyapatite plasma-sprayed coatings
作者： M. P. Ferraz , M. H. Fernandes , A. Trigo Cabral , J. D. Santos , F. J. Monteiro
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摘要: Human osteoblastic bone marrow cells were cultured for periods of up to 28 days in control conditions and on the surface a glass reinforced hydroxyapatite composite (HA/G1) commercial (HA) plasma-sprayed coatings, "as-received" condition after immersion treatment culture medium 21 days. Cultures characterized total protein content alkaline phosphatase activity. Scanning electron microscope analyses performed cultures, seeded materials incubated absence cells. Culture media analyzed ionized calcium phosphorus concentrations throughout incubation period. Immersion HA/G1 HA coatings resulted significant alterations levels medium, leading modifications. However, material samples showed differences pattern variation these species. Cell proliferation was observed composite, but cell mediated formation mineral deposits not proved. In contrast, hardly supported growth. Previously immersed evidence biological deposits. presented better characteristics growth as behavior closer that cultures.
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C. J KIRKPATRICK, M WAGNER, H KOHLER, F BITTINGER, M OTTO, C. L KLEIN, The cell and molecular biological approach to biomaterial research: a perspective. Journal of Materials Science: Materials in Medicine. ,vol. 8, pp. 131- 141 ,(1997) , 10.1023/A:1018567018688
S BEST, B SIM, M KAYSER, S DOWNES, The dependence of osteoblastic response on variations in the chemical composition and physical properties of hydroxyapatite. Journal of Materials Science: Materials in Medicine. ,vol. 8, pp. 97- 103 ,(1997) , 10.1023/A:1018558816871
Paul Ducheyne, Shulamith Radin, Linda King, The effect of calcium phosphate ceramic composition and structure on in vitro behavior. I. Dissolution Journal of Biomedical Materials Research. ,vol. 27, pp. 25- 34 ,(1993) , 10.1002/JBM.820270105
E. Lugscheider, M. Knepper, B. Heimberg, A. Dekker, C. J. Kirkpatrick, Cytotoxicity investigations of plasma sprayed calcium phosphate coatings Journal of Materials Science: Materials in Medicine. ,vol. 5, pp. 371- 375 ,(1994) , 10.1007/BF00058966
Christel P. A. T. Klein, P. Patka, H. B. M. van der Lubbe, J. G. C. Wolke, K. de Groot, Plasma-sprayed coatings of tetracalciumphosphate, hydroxyl-apatite, and alpha-TCP on titanium alloy: an interface study Journal of Biomedical Materials Research. ,vol. 25, pp. 53- 65 ,(1991) , 10.1002/JBM.820250105
J. D. de Bruijn, C. A. van Blitterswijk, J. E. Davies, Initial bone matrix formation at the hydroxyapatite interface in vivo Journal of Biomedical Materials Research. ,vol. 29, pp. 89- 99 ,(1995) , 10.1002/JBM.820290113
C. Maniatopoulos, J. Sodek, A.H. Melcher, Bone formation in vitro by stromal cells obtained from bone marrow of young adult rats. Cell and Tissue Research. ,vol. 254, pp. 317- 330 ,(1988) , 10.1007/BF00225804
M NAGASE, Y ABE, M CHIGIRA, E UDAGAWA, Toxicity of silica-containing calcium phosphate glasses demonstrated in mice Biomaterials. ,vol. 13, pp. 172- 175 ,(1992) , 10.1016/0142-9612(92)90067-X
J. Amedee, R. Bareille, R. Jeandot, L. Bordenave, M. Remy, F. Rouais, Ch. Baquey, Evaluation of cell colonization on biomaterials: preventing cell attachment to plastic containers. Biomaterials. ,vol. 15, pp. 1029- 1031 ,(1994) , 10.1016/0142-9612(94)90086-8
J. H. Chern Lin, M. L. Liu, C. P. Ju, Morphologic variation in plasma-sprayed hydroxyapatite-bioactive glass composite coatings in Hank's solution. Journal of Biomedical Materials Research. ,vol. 28, pp. 723- 730 ,(1994) , 10.1002/JBM.820280609