Immortalization of bone marrow-derived human mesenchymal stem cells by removable simian virus 40T antigen gene: analysis of the ability to support expansion of cord blood hematopoietic progenitor cells.

摘要: Human marrow-derived mesenchymal stem cells (MSC), which have the potential to differentiate into tissues, such as bone, cartilage, adipose and bone marrow stroma, were transduced with a retroviral vector carrying simian virus 40 large T antigen, hygromycin-resistant gene herpes simplex thymidine kinase gene, that can be excised by Cre/loxP site-specific recombination. This resulted in establishment of an MSC cell line, HMSC-1, retained original surface characteristics differentiation potential, exhibited higher proliferative capacity than parental cells. HMSC-1 expressed mRNAs BMP-4, Jagged-1, SCF are known promote hematopoiesis. CB CD34+ hematopoietic progenitor (HPC) cultured on layer showed high expansion CD34+CD38- immature HPC, capable reconstituting human hematopoiesis non-obese diabetic/severe combined immunodeficient disease (NOD/SCID) mice. line may value for developing strategies ex vivo HPC.