In vitro intestinal bioavailability of arsenosugar metabolites and presystemic metabolism of thio-dimethylarsinic acid in Caco-2 cells.

摘要: Whereas inorganic arsenic is classified as a human carcinogen, risks to health related the presence of arsenosugars in marine food are still unclear. Since studies indicate that metabolites contribute induced carcinogenicity, risk assessment for should also include toxicological characterization their respective metabolites. Here we assessed intestinal bioavailability arsenosugar oxo-DMAAV, thio-DMAAV, oxo-DMAEV, thio-DMAEV and thio-DMAV relation arsenite Caco-2 barrier model. caused disruption at concentrations ≥10 μM, all other did not cause leakage, even when applied 50 times higher than thio-DMAV. The transfer point strong thio-DMAEV, whereas thio-DMAAV oxo-DMAEV passed vitro only very small extent. Detailed influx efflux cross most likely by passive diffusion (paracellular) facilitated (transcellular) transport. LC-ICP-QMS based speciation during experiments demonstrate itself across suggest metabolism using model its oxygen-analogue DMAV. In case no was observed. summary two showed similar arsenite, about 10-fold reported (Leffers et al., Mol. Nutr. Food Res., 2013, DOI: 10.1002/mnfr.201200821) same Thus, presystemic might strongly impact after intake therefore be considered assessing consumption arsenosugar-containing food.