CDP phosphotransferase activity in spinach intact chloroplasts: Possible involvement of nucleoside diphosphate kinase II
作者： Lucien Bovet , Marlyse Meylan-Bettex , Thomas Eggman , Enrico Martinoia , Paul-André Siegenthaler
DOI: 10.1016/S0981-9428(00)80094-7
关键词:
摘要: Abstract Nucleotides formation after addition of [γ 32 P]-ATP has been analysed in isolated chloroplasts the presence exogenous CDP, UDP and GDP. The highest level phosphotransfer was observed on CDP 10 min incubation. Interestingly, phosphorylation increase chloroplastic organello correlated with time-dependent dephosphorylation a 18-kDa polypeptide, thereby indicating that major endogenous phosphorylated NDP, is likely to be potent vivo substrate for this phosphoprotein. polypeptide immunoprecipitated antibodies directed against human nucleoside diphosphate kinase (NDPK) A/B spinach NDPK-II, both belonging ubiquitous family NDPKs (EC 2.7.4.6). Using recombinant we could not show preference vitro, suggesting either most available NDPK-II intact or factor modulates enzyme affinity substrates vivo.
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sci-hub.se 参考文章(31)
E. H. Postel, NM23/Nucleoside Diphosphate Kinase as a Transcriptional Activator of c-myc Current Topics in Microbiology and Immunology. ,vol. 213, pp. 233- 252 ,(1996) , 10.1007/978-3-642-61109-4_11
R.E. Parks, R.P. Aganwal, 9 Nucleoside Diphosphokinases The Enzymes. ,vol. 8, pp. 307- 333 ,(1973) , 10.1016/S1874-6047(08)60069-4
N J MacDonald, A De la Rosa, M A Benedict, J M Freije, H Krutsch, P S Steeg, A serine phosphorylation of Nm23, and not its nucleoside diphosphate kinase activity, correlates with suppression of tumor metastatic potential Journal of Biological Chemistry. ,vol. 268, pp. 25780- 25789 ,(1993) , 10.1016/S0021-9258(19)74458-5
Hideo Baba, Takeshi Urano, Kazuya Okada, Koichi Furukawa, Eiichi Nakayama, Hajime Tanaka, Katsuro Iwasaki, Hiroshi Shiku, None, Two Isotypes of Murine nm23/Nucleoside Diphosphate Kinase, nm23-M1 and nm23-M2, Are Involved in Metastatic Suppression of a Murine Melanoma Line Cancer Research. ,vol. 55, pp. 1977- 1981 ,(1995)
Jens L�beck, J�rgen Soll, Nucleoside diphosphate kinase from pea chloroplasts: purification, cDNA cloning and import into chloroplasts Planta. ,vol. 196, pp. 668- 673 ,(1995) , 10.1007/BF01106759
A.M. Gilles, E. Presecan, A. Vonica, I. Lascu, Nucleoside diphosphate kinase from human erythrocytes. Structural characterization of the two polypeptide chains responsible for heterogeneity of the hexameric enzyme. Journal of Biological Chemistry. ,vol. 266, pp. 8784- 8789 ,(1991) , 10.1016/S0021-9258(18)31515-1
P.-A. Siegenthaler, L. Bovet, A unique protein-kinase activity is responsible for the phosphorylation of the 26- and 14-kDa proteins but not of the 67-kDa protein in the chloroplast envelope membranes of spinach Planta. ,vol. 190, pp. 231- 240 ,(1993) , 10.1007/BF00196616
Yukiko Sasaki, Kazumasa Sekiguchi, Yukio Nagano, Ryuichi Matsuno, Detection of small GTP-binding proteins in the outer envelope membrane of pea chloroplasts FEBS Letters. ,vol. 293, pp. 124- 126 ,(1991) , 10.1016/0014-5793(91)81166-6
Mihiro Yano, Sachie Mori, Yasuharu Niwa, Masahiro Inoue, Hiroshi Kido, Intrinsic nucleoside diphosphate kinase-like activity as a novel function of 14-3-3 proteins. FEBS Letters. ,vol. 419, pp. 244- 248 ,(1997) , 10.1016/S0014-5793(97)01469-5
F Kessler, G Blobel, H. Patel, D. Schnell, Identification of two GTP-binding proteins in the chloroplast protein import machinery Science. ,vol. 266, pp. 1035- 1039 ,(1994) , 10.1126/SCIENCE.7973656