Pathological investigation of sentinel lymph nodes.

摘要: The sentinel lymph-node procedure enables selective targeting of the first draining lymph node, where initial metastases will form. A negative node (SN) predicts absence tumour in other regional nodes with high accuracy. This means that case a SN, dissection is no longer necessary. Besides saving costs, this prevent many side-effects as result dissection. task pathologist to screen SNs for metastases. To end, several techniques are available such standard histo- and cytopathological techniques, immunohistochemistry, flow cytometry, molecular biological techniques. These methods explained their sensitivity detecting SN discussed. Some these also appear be useful intra-operative evaluation SNs. protocol detection consists extensive histopathological investigation including step H&E stained sections immunohistochemistry. Intra-operative frozen-section analysis has been shown reliable breast-cancer axillary nodes. In setting, imprint cytology can used but its additional value frozen section not yet clear. Further studies necessary establish role sophisticated reverse transcription polymerase chain reaction (RT-PCR) cytometry too low purpose.