Mechanism of Action of Showdomycin:Part I. Effect of Showdomycin on the Syntheses of Nucleic Acids and Proteins in Escherichia coli K-12

摘要: Showdomycin, 2-β-d-ribofuranosylmaleimide, inhibited the incorporation of amino acids and purine pyrimidine bases into macromolecules in E. coli K-12 cells at low concentrations. The inhibitory action showdomycin could be reversed by addition a nucleoside or sulfhydryl compound. In marked contrast to common nucleosides, pseudouridine showed no such effect. This may indicate that N-glycosyl linkage is structural requirement for its reversing activity on showdomycin.N-Ethylmaleimide, which has similarity showdomycin, as well showdomycin. N-ethylmaleimide, however, was not nucleoside. there difference mechanism between N-ethylmaleimide