Primary structure of the RAD52 gene in Saccharomyces cerevisiae.

摘要: The RAD52 gene of Saccharomyces cerevisiae, which is involved in genetic recombination and DNA repair, was cloned by transformation rad52-1 mutant cells to methyl methanesulfonate resistance with BamHI fragments Rad+ genomic inserted into the Escherichia coli-S. cerevisiae shuttle vector YRp7. A plasmid carrying a 2.0-kilobase fragment found partially complement sensitivity mutant. By using this as hybridization probe, that fully complemented isolated, carries 3.3-kilobase SalI containing most fragment. Analysis nucleotide sequence revealed presence large open reading frame 1,512 nucleotides. has single-base change frame, leads an amino acid substitution. mRNA synthesized yeast S1 mapping technique disclosed possible transcription initiation termination points suggested formation product without splicing transcript.