Specific binding of melatonin in bovine brain.
作者： DANIEL P. CARDINALI , MARÍA I. VACAS , EDUARDO ESTÉiVEZ BOYER
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摘要: High affinity binding of melatonin in crude membrane preparations bovine brain was examined by a rapid filtration procedure through Whatman GFB paper. Melatonin to medial basal hypothalamic (MBH) membranes attained its maximum at the first, third, and fifth hours incubation at. 37,18, 0 C, respectively. Specific linear up 3 mg protein, thermolabile, decreased after with trypsin; it also pH dependent, being observed 7.4. affected variety ionic manipulations; inhibited 55% 62% addition 10 mM KC1 120 NaCl, respectively, increased 40% 50% 4 or 6 CaCl2. 25% 1.25 MgCl2, whereas depressed higher concentrations. Among various regions studied, maximal MBH; indole occipital cerebellar cortexes 73% 34% that MBH. Subcellular frac...
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