hsa-miR-20b-5p and hsa-miR-363-3p Affect Expression of PTEN and BIM Tumor Suppressor Genes and Modulate Survival of T-ALL Cells In Vitro

摘要: T-cell acute lymphoblastic leukemia (T-ALL) is an aggressive malignancy arising from T lymphocyte precursors. We have previously shown by miRNA-seq, that miRNAs the mir-106a-363 cluster are overexpressed in pediatric T-ALL. In silico analysis indicated their potential involvement regulation of apoptosis. Here, we aimed to test hypothesis on pro-tumorigenic roles these T-ALL cells vitro. demonstrate, for first time, hsa-miR-20b-5p and hsa-miR-363-3p cluster, when upregulated vitro, protect leukemic apoptosis, enhance proliferation, contribute growth advantage. show, using dual luciferase reporter assays, Ago2-RNA immunoprecipitation, RT-qPCR, Western blots, oncogenic effects might, at least part, be mediated downregulation two important tumor suppressor genes, PTEN BIM, targeted both miRNAs. Additionally, demonstrate cooperative simultaneous inhibition as compared single postulate might serve oncomiRs T-ALL, contributing post-transcriptional repression key suppressors, BIM.