Regulation of outer membrane porin protein synthesis in Escherichia coli K-12: ompF regulates the expression of ompC.

摘要: The relative amounts of the OmpF and OmpC proteins in outer membrane Escherichia coli K-12 are affected differentially by high concentrations substances like sucrose culture media such a manner that decrease amount protein appears to be compensated for reciprocal increase protein. When an ompF mutation was introduced, synthesis became almost independent occurred at fully induced level even absence sucrose. On other hand, introduction ompC did not affect sucrose-dependent profile synthesis. effect also examined with ompC-lac fusion strain, which expression beta-galactosidase is under control promoter. coded ompF+ ompF- strains essentially same as protein, being dependent strain sucrose-independent mutant. From these results we conclude medium primarily regulates gene expression, turn transcriptional level. This sequential regulatory mechanism discussed relation function ompB locus.