Thromboxane A2 receptor mediated activation of the mitogen activated protein kinase cascades in human uterine smooth muscle cells
作者： Sinead M Miggin , B.Therese Kinsella
DOI: 10.1016/S0167-4889(01)00103-3
关键词:
摘要: Both thromboxane (TX) A2 and 8-epi prostaglandin (PG) F2α have been reported to stimulate mitogenesis of vascular smooth muscle (SM) in a number species. However, TXA2 8-epiPGF2α mediated mitogenic signalling has not studied detail human SM. Thus, using the uterine ULTR cell line as model, we investigated receptor (TP) cultured SMCs. TP agonist U46619 elicited time concentration dependent activation extracellular signal regulated kinase (ERK)s c-Jun N-terminal (JNK)s cells. Whereas antagonist SQ29548 abolished signalling, it only partially inhibited ERK JNK induced activations were by protein (PK) C, PKA phosphoinositide 3-kinase inhibitors GF109203X, H-89 wortmannin, respectively, but unaffected pertussis toxin. In addition, cells involves transactivation epidermal growth factor (EGF) receptor. humans, signals through two distinct isoforms. investigating involvement isoforms both TPα TPβ independently directed embryonic kidney (HEK) 293 over-expressing individual contrast that which occurred cells, HEK providing further evidence may alternative receptors, addition TPs,
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