Metal free columns for determination of deoxynucleotide monophosphate by liquid chromatography/mass spectrometry and application to oligonucleotide.
作者： Sachie Shibayama , Hiroshi Sakamaki , Taichi Yamazaki , Akiko Takatsu
DOI: 10.1016/J.CHROMA.2015.06.033
关键词:
摘要: Abstract We have developed a highly sensitive method for the analysis of deoxynucleotide monophosphates (dNMPs), which involves use liquid chromatography/mass spectrometry (LC/MS) and new metal-free column. The column solves problem that phosphate group in dNMPs interacts with metal portion device or After optimization analytical conditions, limits detection (LODs) were from 5.4 ng/g to 6.3 ng/g. Those values 10 times lower than LODs previous methods. applied determination base composition quantification 20-mer oligonucleotide. Despite very small sample amount 14.5 ng, we able determine composition, result was consistent theoretical values. also quantify mass fraction oligonucleotide 8.2% expanded uncertainty ( k = 2). By means method, analyze high sensitivity as well despite sample.
sci-hub.se 参考文章(18)
Handbook of Analysis of Oligonucleotides and Related Products CRC Press. ,(2011) , 10.1201/B10714
Gavin O'Connor, Carol Dawson, Alison Woolford, Kenneth S. Webb, Tim Catterick, Quantitation of oligonucleotides by phosphodiesterase digestion followed by isotope dilution mass spectrometry: Proof of concept Analytical Chemistry. ,vol. 74, pp. 3670- 3676 ,(2002) , 10.1021/AC0255375
Shin-ichiro Fujii, Kazumi Inagaki, Koichi Chiba, Akiko Takatsu, Quantification of phosphorus in DNA using capillary electrophoresis hyphenated with inductively coupled plasma mass spectrometry. Journal of Chromatography A. ,vol. 1217, pp. 7921- 7925 ,(2010) , 10.1016/J.CHROMA.2010.10.066
Tomoya Kinumi, Tomohiro Narukawa, Shin-Ichiro Fujii, Sakae Eyama, Mika Saeki, Akiko Takatsu, Quantification of oligonucleotide containing sequence failure product: comparison of isotope dilution mass spectrometry with other quantification methods. European Journal of Mass Spectrometry. ,vol. 15, pp. 399- 407 ,(2009) , 10.1255/EJMS.986
Nan-Sook Hong, Lian Hua Shi, Ji Seon Jeong, Inchul Yang, Sook-Kyung Kim, Sang-Ryoul Park, Rapid and accurate determination of deoxyribonucleoside monophosphates from DNA using micellar electrokinetic chromatography with a cationic surfactant additive Analytical and Bioanalytical Chemistry. ,vol. 400, pp. 2131- 2140 ,(2011) , 10.1007/S00216-011-4915-5
Carol E. Donald, Peter Stokes, Gavin O’Connor, Alison J. Woolford, A comparison of enzymatic digestion for the quantitation of an oligonucleotide by liquid chromatography-isotope dilution mass spectrometry. Journal of Chromatography B. ,vol. 817, pp. 173- 182 ,(2005) , 10.1016/J.JCHROMB.2004.11.058
Feng LIANG, Yu-Lan LI, Wen-Wei LU, Ming-Guang TAN, Gui-Lin ZHANG, Yan LI, Analysis of Deoxyribonucleotide in Plasmid Deoxyribonucleic Acid Using High Performance Liquid Chromatography-Inductively Coupled Plasma-Mass Spectrometry Chinese Journal of Analytical Chemistry. ,vol. 36, pp. 1097- 1100 ,(2008) , 10.1016/S1872-2040(08)60060-2
Claire L. Camp, Barry L. Sharp, Helen J. Reid, John Entwisle, Heidi Goenaga-Infante, Analysis of mono-phosphate nucleotides as a potential method for quantification of DNA using high performance liquid chromatography–inductively coupled plasma-mass spectrometry Analytical and Bioanalytical Chemistry. ,vol. 402, pp. 367- 372 ,(2012) , 10.1007/S00216-011-5347-Y
Lianhua Dong, Ying Meng, Jing Wang, Yingying Liu, Evaluation of droplet digital PCR for characterizing plasmid reference material used for quantifying ammonia oxidizers and denitrifiers. Analytical and Bioanalytical Chemistry. ,vol. 406, pp. 1701- 1712 ,(2014) , 10.1007/S00216-013-7546-1
Hiroshi Sakamaki, Takeharu Uchida, Lee Wah Lim, Toyohide Takeuchi, Evaluation of column hardware on liquid chromatography-mass spectrometry of phosphorylated compounds. Journal of Chromatography A. ,vol. 1381, pp. 125- 131 ,(2015) , 10.1016/J.CHROMA.2014.12.088