Evaluation of column hardware on liquid chromatography-mass spectrometry of phosphorylated compounds.
作者： Hiroshi Sakamaki , Takeharu Uchida , Lee Wah Lim , Toyohide Takeuchi
DOI: 10.1016/J.CHROMA.2014.12.088
关键词:
摘要: Abstract The influences of column hardware, such as chromatographic tubes and frits, on liquid chromatography–mass spectrometry (LC–MS) analysis phosphorylated compounds were evaluated. signal to noise ratio (S/N) the intensity flavin adenine dinucleotide (FAD) using a glass lined tube polyethylene frit (GL–PE) was approximately 170 90 times higher, respectively, than those conventional stainless steel (S–S) column. In addition, retention time FAD GL–PE shortest compared other columns. Interaction between metal ions in flow path S–S stronger that them Thus, low. Since specific surface area pair frits 70 larger (150 mm × 2.1 mm), found have more effective improvement S/N well tubes, when analyzed by LC–MS. When evaluated LC–MS(/MS) column, increased.
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