Studies of Macromolecular Interaction by Sedimentation Velocity

摘要: As our knowledge expands and new systems are investigated, it becomes clear that elucidating precise biochemical regulatory mechanisms requires detailed understanding of macromolecular assembly processes. For example, the regulation gene expression involves an intricate network protein-protein protein-nucleic acid interactions mechanism some allosteric enzymes is linked to subunit assembly. In order define these one needs information on identities proteins in complex, affinities for each other effects regulators formation complexes. A direct way studying monitor resulting changes mass as a consequence One methods enables directly macromolecules transport technique. Among sedimentation analysis technique choice because sound fundamental principles which method based its resolving power. Excellent reviews applications equilibrium self-associations heteropolymers included Part I this volume. chapter focus velocity. advantages velocity over speed analysis, e.g., run can be completed within hour whereas experiments may take much longer. Hence, if biological sample unstable study system have conducted using However, larger amount will required than measurements, calf brain tubulin rabbit muscle phosphofructokinase require few mg protein curve coefficient vs concentration.