Delivery of fluorophores by calcium phosphate-coated nanoliposomes and interaction with Staphylococcus aureus biofilms

摘要: The delivery capacity and mechanical stability of calcium phosphate (CaP) coated 1,2-dioleoyl-sn-glycero-3-phosphate (DOPA) liposomes free adsorbed on bacterial surface was investigated introducing either acridine orange (AO) or 5,10,15,20-Tetrakis(1-methyl-4-pyridinio)porphyrin (TMP) in the aqueous core liposomes. obtained nanomaterials were thoroughly characterized by electron optical microscopy fluorescence techniques. Distribution AO TMP molecules between outer solution demonstrated band shifts broadening excitation-emission matrices modified Stern-Volmer model for quenching. In suspensions, c.a. 40% released to while only a small percentage observed reach liposome surface. nanoliposomes adhesion leaking fluorophore Staphylococcus aureus (S. aureus) biofilms further evaluated. A close interaction S. biofilm evidenced TEM SEM imaging. Epifluorescence experiments that CaP-coated have good staining capability after two hours incubation with liposomes, thus supporting an important release fluorophores when contact biofilm. Altogether, results strongly suggest are capable activating drug presence smears. studies herein presented, indicate potential vehicles selective drugs biofilms, as is case singlet oxygen photosensitizer TMP, well known photodynamic antibacterial agent.