Contacting co-culture of human retinal microvascular endothelial cells alters barrier function of human embryonic stem cell derived retinal pigment epithelial cells
作者： H. Skottman , J. Muranen , H. Lähdekorpi , E. Pajula , K. Mäkelä
DOI: 10.1016/J.YEXCR.2017.08.004
关键词:
摘要: Here we evaluated the effects of human retinal microvascular endothelial cells (hREC) on mature embryonic stem cell (hESC) derived pigment epithelial (RPE) cells. The hESC-RPE (Regea08/017, Regea08/023 or Regea11/013) and hREC (ACBRI 181) were co-cultured opposite sides transparent membranes for up to six weeks. Thereafter barrier function, small molecule permeability, localization RPE marker proteins, cellular fine structure, growth factor secretion evaluated. After co-culture, specific CRALBP von Willebrand appropriately localized. In addition, general morphology, pigmentation, structure unaffected. Co-culture increased function cells, detected both with TEER measurements cumulative permeability FD4 - although differences varied among lines. Co-culturing significantly altered VEGF PEDF secretion, but again line specific. results this study showed that co-culture affects functionality. revealed drastic differences, most notably in secretion. This model has potential be used as an vitro outer blood-retinal drug testing.
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