Programmable electrophoresis with integrated and multiplexed control
作者： Philip Serwer , Frederick J. Dunn
DOI:
关键词:
摘要: A user programmable device for horizontal gel eletrophoresis is provided. This can both accurately and programmably control: (a) the angle between electrical field by use of a rotatable bed, (b) magnitude field, (c) temperature. The present invention microprocessor-based contol board which sends control signals to: motor attached to source elecrical potential Peltier devices sides buffer chambers horizontally displaced from bed. particularly appropriate lowering cost, footprint, reducing heat dissipated, maintaining close tolerances electric gradients temperature, exploring using thus far untried modes variation. It thereby suitable analyzing large linear DNA, open circular DNA and/or DNA-protein complexes. Control several disks, power supplies temperature cells one (multiplexing) an option.
google.it 参考文章(63)
P. Serwer, D. Louie, A hybrid mode of rotating gel electrophoresis for separating linear and circular duplex DNA. Applied and theoretical electrophoresis : the official journal of the International Electrophoresis Society. ,vol. 1, pp. 169- 173 ,(1989)
Philip Serwer, Template for forming multiple gel tracks within a single electrophoretic gel frame ,(1980)
Ellis Sidney G, Stevenson Peter C, Method and apparatus for separation of colloids in a colloid solution ,(1951)
Uli Schwarz, Robert Neukirchen, Method and apparatus for performing uni- and bi-dimensional micro-gel electrophoresis ,(1980)
Philip Serwer, [8] Use of gel electrophoresis to characterize multimolecular aggregates Methods in Enzymology. ,vol. 130, pp. 116- 132 ,(1986) , 10.1016/0076-6879(86)30010-7
Norman L. Anderson, Apparatus for electrophoresis separation ,(1977)
P Serwer, W E Masker, J L Allen, Stability and in vitro DNA packaging of bacteriophages: effects of dextrans, sugars, and polyols. Journal of Virology. ,vol. 45, pp. 665- 671 ,(1983) , 10.1128/JVI.45.2.665-671.1983
Cassandra L. Smith, Charles R. Cantor, Purification, specific fragmentation, and separation of large DNA molecules. Methods in Enzymology. ,vol. 155, pp. 449- 467 ,(1987) , 10.1016/0076-6879(87)55030-3