Probe and primer sensitized by locking nucleic acid and used for detecting C677T mutation of MTHFR gene, kit and detection method

摘要: The invention discloses a probe and primer sensitized by locking nucleic acid used for detecting C677T mutation of an MTHFR gene, kit detection method. By means the property that can flexibly modify TaqMan probe, proper position gene is modified to increase specificity. Fluorescence quantitative PCR amplification carried out on sample genome DNA obtained in step (1) order measure Ct value wild mutant probe; positive checked when smaller than 35 recurring numbers; only detected be positively homozygotic type CC; both CT heterozygote.