Preservation of vein allograft viability during long-term storage.

摘要: Background: The aim of this study was to compare the vein allograft viability following cryopreservation with that remaining after prolonged refrigerated storage. Materials and Methods: Great saphenous biopsies had been cryopreserved, samples were divided into two matched groups stored in tissue culture medium for 42 days at +4°C, either or without regular replacement. Each biopsied assayed every third day by methyltetrazolium reduction assay. Viability indexes allografts harvested from brain-dead multi-organ donors cadavers whose warm ischemic periods maximally 24 h also compared. Results: Vein +4°C showed a similar (58.9 ± 1.2%) cryopreserved veins (59.7 2.3%). This true even when thawed subjected 3 post-thaw incubation under presumably favorable conditions (58.7 1.6%). There no index difference between replaced not specimens different donor groups. Conclusions: Long-term storage is valuable option banking practice. Sufficient amounts can be procured donors.