A Simplified Technique for the Cryopreservation of Vein Allografts
作者： AC Ruddle , SJ George , WJ Armitage , EL Alexander , DC Mitchell
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摘要: Abstract Objectives: we assessed the effects of cryopreservation on smooth-muscle cell injury in human vein. Materials and methods: long saphenous vein was collected during surgery cryopreserved. Smooth-muscle damage after thawing by situ detection fragmented DNA. The presence cryoprotectant (10% dimethyl sulphoxide, DMSO), cooling warming rates, rate removal were examined. Results: control veins exhibited 8.5% (95% confidence interval (CI) 4.7 to 13.4%,n=13) cells compared with 27.7% CI 23.2 32.4%, n=115) frozen 10% DMSO (p=0.001). In DMSO, independent rates (p=0.72) (p=0.45). dilution remove also had no effect (p=0.64). absence cryoprotectant, doubled approximately 50% slow rather than rapid (p=0.01). Conclusion: rate, a has little damage, provided that tissue is warmed rapidly. Slow warming, causes substantial damage. These results suggest simplified methods are feasible.
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