Binding and cytotoxicity characteristics of the bispecific murine monoclonal antibody 2B1.

摘要: Bispecific monoclonal antibodies (BsmAb) with specificity for tumor Ag and effector cell trigger molecules have been shown to redirect the cytotoxicity of several peripheral blood mononuclear populations against relevant tumor. The BsmAb, 2B1, binds extracellular domain c-erbB-2 gene product HER2/neu proto-oncogene CD16. In this report, binding cytotoxic characteristics 2B1 are presented. Maximal saturation PBL expressing SK-OV-3 cells occurred in 1 microgram/ml concentration range. However, substantial lysis potentiation was observed at 1000-fold lower BsmAb concentrations. Optimal obtained when PBL, target were continuously coincubated. When franked washed, added labeled targets, substantially less observed. These results suggest that best way therapeutically exploit attributes may be obtain continuous exposure Approaches based on franking not warranted.