Zebularine Promotes Hepatic Differentiation of Rabbit Bone Marrow Mesenchymal Stem Cells by Interfering with p38 MAPK Signaling.

摘要: Demethylating agent zebularine is reported to be capable of inducing differentiation stem cells by activation methylated genes, though its function in hepatocyte unclear. p38 signal pathway involved hepatocytes and regulating DNA methyltransferases 1 (DNMT1) expression. However, little known about the impact on bone marrow mesenchymal (BMMSCs) signaling during hepatic differentiation. The present study investigated effects rabbit BMMSCs, as well role DNMT1 differentiation, with aim developing a novel strategy for improving derivation hepatocytes. BMMSCs were treated at concentrations 10, 20, 50, 100 μM presence growth factor; changes levels hepatic-specific alpha-fetoprotein albumin detected determined RT-PCR, WB, immunofluorescence staining. Expression phosphorylated urea production ICG metabolism was also analyzed. Zebularine 50 μM could not affect cell viability after 48 h. treatment leads an inhibition DNMT activity increase proteins vitro; addition induced expression metabolism. activated simulated HGF; facilitated synthesis cells. restrained which HGF. Therefore, this demonstrated that exhibited terminal vitro association least partially participates zebularine-induced BMMSCs.